Figure 2.

eGFP expression in AAV-treated ROs differentiated for 80 and 300 days

(A) Confocal imaging of AAV-treated organoids expressing eGFP (green). Left, brightfield; middle, x-y projection; right, y-z projection. Scale bars: 500 μm.

(B–D) (B) Quantification of mean eGFP fluorescence in day 80 and day 300 ROs exposed for 1, 2, or 3 days to the respective AAV (1 × 10¹⁰ virus genomes/well) and quantified after 7 days. Statistics represent the comparison between AAV for the same RO age with scAAV2.7m8 (^∗) and with scShh10 (#). Kinetics of eGFP for (C) day 80 ROs and (D) day 300 ROs. (B–D) n = 17–18 separate ROs per condition, mean + SEM.

See also Figures S2 and S3.