Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 20;11:18641. doi: 10.1038/s41598-021-97535-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Design and analysis of the CTA1-DD-RBF protein. (A) Domain structure of the CTA1-DD-RBF protein. Mutations (Val to Leu at residue 207 and Ser to Phe mutation at residue 190; disulfide between residues 290 and 155) and disulfide bonds 149 to 458 were incorporated into the F protein and are indicated by vertical black lines. CTA1-DD-RBF contains the CTA1 subunit of cholera toxin (CT), two immunoglobulin (Ig) binding domains (DD) of staphylococcal protein A and hRSV F protein (residues 26–105 and 146–513) with a T4 fibritin trimerization motif (foldon) and a variable linker, GSGSG. (B) CTA1-DD-RBF proteins digested by thrombin on SDS-PAGE: Marker: protein markers; Lane 1: CTA1-DD-RBF purified using a HisTrap FF column after digested by thrombin; Lane 2, CTA1-DD-RBF purified by HisTrap FF columns before digested by thrombin. (C) Negative stain electron microscopy of CTA1-DD-RBF. The CTA1-DD-RBF was highly homogenous. Bar = 50 nm.