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. 2021 Sep 20;12:5535. doi: 10.1038/s41467-021-25843-6

Fig. 1. The E1RF helicase complex.

Fig. 1

a Domain organisation of Bovine Papillomavirus (BPV) E1. b A schematic representation of the E1RF complex on the fork sequence used. c A homogenous fraction of the E1RF complex purified by gel filtration was used for cryo-EM. The calibration markers are thyroglobulin, 669 kDa, ferritin, 440 kDa, catalase 240 kDa and conalbumin, 75 kDa. d The unwinding activity of the E1 protein (50, 100, 200 and 400 nM E1) on the replication fork substrate used to generate E1RF and comparison with substrates without 5′ or 3′ ssDNA tails (100 nM E1, n = 3 independent experiments; shown mean values with error bars ± SD).