Fig. 5. Histone Kinic leads to higher chromatin accessibility and promotes gene transcription.
a, b Histone Kinic induces chromatin accessibility. a HepG2 cells were treated with or without 10 mM INH for 6 h, nucleosomal DNA was extracted and analyzed by MNase sensitivity assay (left). “CON” indicates untreated HepG2 cells; “INH” indicates isoniazid treated HepG2 cells, MW: Molecular Weight. Quantifications of lane signal intensity (right, upper bands), data presented are the mean ± SEM from three biological replicates (n = 3), as determined by unpaired two-tailed Student’s t-test b Relative mRNA expression of Sat-2 in HepG2 cells treated with or without 10 mM INH for 12 h and determined by real-time RT-PCR assay, “CON” indicates untreated HepG2 cells; “INH” indicates isoniazid treated HepG2 cells, data presented are the mean ± SEM from three biological replicates (n = 3), as determined by unpaired two-tailed Student’s t-test. c Histone Kinic upregulates 313 genes and downregulates 121 genes. Volcano plot analysis of pairwise comparison of RNA-seq results from HepG2 cells with or without 10 mM INH for 12 h (n = 3 samples, values were expressed as mean ± SEM). d KEGG pathway analysis of mRNA levels elevated genes. Some genes involved in each pathway are labelled. e Real-time RT-PCR analysis of the genes labelled in KEGG pathway analysis. Relative expression is normalized to GAPDH, “CON” indicates untreated HepG2 cells; “INH” indicates isoniazid treated HepG2 cells, data presented are the mean ± SEM from three biological replicates (n = 3), as determined by unpaired two-tailed Student’s t-test.