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. 2021 Sep 20;11:18636. doi: 10.1038/s41598-021-98061-1

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Vascular space. Whole brain vascular space for (A) males and (B) females were analyzed by a two-way ANOVA and females had significant differences due to genotype (*p < 0.05). Brain region vascular space for (C) males and (D) females were analyzed by repeated measures. Males had a significant interaction between vascular space, diet, and genotype (p < 0.05). There were no brain region differences for females. There were not post-hoc differences. n = Males: E3 LFD = 14, E3 HFD = 7, E4 LFD = 9, E4 HFD = 7; Females: E3 LFD = 9, E3 HFD = 10, E4 LFD = 10, E4 HFD = 9.