Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 20;11:18587. doi: 10.1038/s41598-021-98131-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Exploration of the putative clinical roles of TNFSF4. (A) IHC staining images are shown to clarify different expression patterns (left to right, in sequence, < 25%, 25–75%, and > 75%). A lymph node slide was set as a positive control, and an unstained slide was set as a negative control. (B) High RNA expression of TNFSF4 was universally identified in breast carcinoma, with testing and calculation based on FPKM, and the cutoff line is labeled, which was used for clinical predictions. (C,D) Higher TNFSF4 expression pointed to poorer survival outcomes. (E,F) Flow cytometry with FACSAria sorting was applied to isolate stem cells from ZR75-1, MCF-7, and MM-231 cells. (G,H) Stem cells with a CD44⁺/24⁻ or ALDH1A1⁺ phenotype were identified and isolated, and the TNFSF4 expression patterns in different cell lines were checked to illustrate the increased expression.