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. 2021 Sep 20;7:254. doi: 10.1038/s41420-021-00643-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Immunofluorescence analysis of LC3 to evaluate autophagy in N2A cells. b–e The protein levels of P62 (c), LC3 (d), and Beclin-1 (e) assessed by western blot in N2A cells subjected to MPP⁺ treatment with or without DHF. f–h The protein levels of P62 (g) and LC3 (h) assessed by western blot in MPP⁺-treated N2A cells with or without co-administration of DHF and CQ or 3-MA. Data are expressed as mean ± SEM. *P < 0.05.