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. 2021 Sep 20;12:5547. doi: 10.1038/s41467-021-25865-0

Fig. 1. Identification and characterization of the phenotypic signatures of the new IRs (LAG-3, TIM-3, and TIGIT) in human ccRCC tumours.

Fig. 1

a–c Automated cell-by-cell segmentations and single-cell counts of human ccRCC tumours immunolabelled for LAG-3 (a), TIM-3 (b), and TIGIT (c). Zoomed-in images of the indicated boxed regions. Scale bars, 50 μm (black) and 20 μm (blue). The experiment was performed one time because of using human sample. d Three-dimensional plots showing positive-stained cell densities for LAG-3, TIM-3, and TIGIT in 105 ccRCC patients. e Hierarchical clustering heatmap (low, blue; high, red) using the positively stained cell densities of LAG-3, TIM-3, and TIGIT in (d). Data were normalized prior to clustering. f Labelling of three-dimensional plots by the inferred cluster types obtained in (e). g, h Kaplan–Meier survival curves for recurrence-free survival (g) and overall survival (h) following surgery in 105 ccRCC patients based on the inferred cluster types. p values were determined with log-rank test. i Percent distribution of the inferred cluster types within IMDC risk groups. p values were determined with a two-tailed Fisher’s exact test (favourable and intermediate (one risk factor) risk groups vs. intermediate (two risk factors) and poor risk groups). Source data are provided as a Source data file.