Fig. 4.

Concept of conditioning of XL-MS distance restraints with HX-MS structural stability measurements.A, a protein complex is analyzed by both HX-MS and XL-MS methods. Both datasets are imported into the IMProv module of the Mass Spec Studio where cross-links are partitioned according to structural dynamics. A three-tiered example is shown. Red-violet-blue color scheme indicates stable, semistable, or unstable structural classes, respectively. Each class informs a scoring function based on a modified distance restraint, in this example presented as the ambiguity of a cross-linked site in space. B, altered distance restraint function based on the slope (left) and offset (right) methods. The slope method is related to the HX stability class