Figure 3.

Destruction of melanoma cells that express various levels of HMW-MAA by CD8⁺CAR-T cells. Cytolytic activity by CD8⁺ CAR-T cells against melanoma cell line A375 (A), CM006 (B), UM004 (C), and UM002B (D) is shown. Estimated numbers of HMW-MAA molecules per each melanoma cell surface are indicated. CD8⁺ T cells transduced with “empty” vector were utilized as control effector cells. All assays were performed in triplicates. Means and SD are calculated for each point, and the error bars are indicated for each mean value. p-values were calculated by Student’s t test, and p-values for statistically significant results are indicated for each point (∗∗∗∗p < 0.0001; ∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05). Data shown are representative of 3 to 5 independent experiments.