Fig. 1.

SEM images demonstrating S. aureus ATCC 6538 biofilm formation on a roughened Si bead following 72 h of growth. (A) Overhead view of a roughened Si bead used for inoculation. (B) High power view showing biofilm following 72h of growth. Note the multi-layered biofilm was predominantly located within the concaved regions of the Si beads. (C) Higher magnification showing a multicell layer. The raw SEM image was digitally colored to help define the cells (purple) and extracellular matrix (yellow) against the roughened Si bead surface (grey).