Table 2.

Growth comparison of Acetobacter sp. CIP 58.66 with glycerol as substrate, at different initial pH

	Shake flask culturesa				Biocatalyst productionb
Initial pH	4.0	4.5	5.0	6.5	5.0
DW (gDW L−1)	0.14 ± 0.01	0.33 ± 0.04	1.14 ± 0.04	0.43 ± 0.02	0.88 ± 0.05
Glycerol consumption (%)c	1.0 ± 0.8	4.6 ± 1.9	16.9 ± 0.7	5.8 ± 0.6	18.6 ± 1.7
Biomass yield (${\text{g}}_{\text{DW}}{\text{g}}_{\text{glycerol}}^{-1}$)	NDd	0.83 ± 0.28	0.69 ± 0.05	0.76 ± 0.06	0.40 ± 0.05
Final pH	4.10 ± 0.02	4.58 ± 0.08	5.38 ± 0.03	6.55 ± 0.03	6.94 ± 0.13
µmax,1 (h−1)	0.10 ± 0.01	0.15 ± 0.004	0.18 ± 0.004	0.11 ± 0.004	0.24 ± 0.02
R2	0.94	0.98	0.99	0.98	0.88
µmax,2 (h−1)	0.03 ± 0.002	0.06 ± 0.002	0.09 ± 0.002	0.07 ± 0.001	0.18 ± 0.01
R2	0.92	0.97	0.99	0.99	0.96

^aComparison at 30.5 h of culture

^bValues at 31 h of culture in bioreactor

^cCalculated as the percentage of glycerol initially supplied

^dCould not be calculated because no significant glycerol consumption occurred