Skip to main content
. 2021 Sep 7;15:713077. doi: 10.3389/fnins.2021.713077

FIGURE 1.

FIGURE 1

Aldh1L1 expression in adult neurogenic niches.(A,B) Immunohistochemical co-staining of Aldh1L1 and GFAP in the adult mouse neurogenic niches (DAPI in blue, GFAP in red, Aldh1L1 in white). (A) Aldh1L1/GFAP double positive astrocytes reside in all DG layers (yellow arrows). Green arrowheads highlight GFAP-positive/Aldh1L1-negative radial processes of adult NSCs in the DG. DG compartment indicated on the left: oML, outer molecular layer; iML, inner molecular layer; GZ, granular zone; SGZ, subgranular zone; scale bar = 20 μm. (B) Aldh1L1/GFAP double positive cells residing in the SEZ (yellow arrows). Green arrowheads point toward GFAP-negative/Aldh1L1-positive cells in the SEZ (scale bar = 20 μm). (C) Crossing scheme showing the genotype of parental mice used to generate experimental Aldh1L1-CreERT2; GFP mice. (D) In order to achieve sufficient recombination, we used different tamoxifen pulses in Aldh1L1-CreERT2; GFP transgenic mice ranging from one to nine injections every 12 h (12 h) and analyzed recombination 2 h post-injection (2 h pi). (E–H) With increasing numbers of tamoxifen injections we observed an increase in the number of recombined astrocytes in the DG of adult Aldh1L1-CreERT2; GFP mice (DAPI in blue, GFP in green, GFAP in red; scale bar = 50 μm).