Quantitative analysis using Image J
(A) Detected cell nuclei after using the above mentioned macro. A separate table with the total number accompanies this image.
(B) Quantification of F4/80-positive cells and the corresponding table for counting (red fluorescence).
(C) Quantification of F4/80-CD206-double positive cells and the corresponding table for counting (green fluorescence).
Scale bar: 50 μm.