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. 2021 Sep 15;2(4):100833. doi: 10.1016/j.xpro.2021.100833

Blocking buffer

Reagent Final concentration Amount
Bovine Serum Albumin 1% (w/v) 400 mg
Fetal Bovine Serum (FBS) 2% (v/v) 800 μL
PBS ∼ 39 mL
Total n/a 40 mL