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. 2021 Sep 12;2021:6610529. doi: 10.1155/2021/6610529

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Copyright © 2021 Na Li et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Analysis of HSP60 phosphorylation at residue Ser70 in human ovarian cancer tissue mitochondrial samples with mass spectrometry and in ovarian cancer cell TOV-21G with immunoprecipitation and western blot. (a) The MS/MS spectrum of phosphopeptide TVIIEQSWGS∗PK (S∗ = phosphoserine) derived from HSP60 in ovarian cancer tissues. (b) HSP60 was immunoprecipitated with an anti-HSP60 antibody, followed by phosphorylation analysis of immunoprecipitated HSP60 with western blot against the anti-phosphoserine antibody at residue Ser70 of HSP60 in ovarian cancer cells TOV-21G.