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. 2021 Sep 7;15:683687. doi: 10.3389/fncel.2021.683687

FIGURE 2.

FIGURE 2

Genotype of MOG-reactive Th1 cells does not contribute significantly to EAE while host genotype determines disease outcome. Ten week-old IL-20RB–/– mice and wild-type mice, male and female, were passively immunized for EAE with 106 cells of either IL-20RB–/– MOG-Th1 or wild-type MOG-Th1 cells (n = 8). Donor to host mouse groups were the following: wild-type → wild-type mice (black circle), IL-20RB–/– → wild-type mice (red circle/black outline), wild-type → IL-20RB–/– mice (black circle/red outline), and IL-20RB–/– → IL-20RB–/– mice (red circle). Clinical score, body weight, highest and cumulative scores, and disease onset from 7 to 28 dpi were analyzed by non-parametric Kruskal–Wallis followed by Dunn’s post hoc test comparing primarily IL-20RB–/– → wild-type mice with wild-type → IL-20RB–/– mice (A–D). Wild-type → wild-type mice (mean ± SEM clinical score: 1.53 ± 0.18, body weight: 21.93 ± 0.25), and IL-20RB–/– → IL-20RB–/– mice (mean ± SEM clinical score: 0.13 ± 0.03, body weight: 26.10 ± 0.28) showed outcomes comparable to wild-type mice and IL-20RB–/– mice with EAE (Figure 1A); but differences in clinical score and body weight between IL-20RB–/– → wild-type mice (mean ± SEM clinical score: 1.66 ± 0.17, body weight: 20.09 ± 0.28), and wild-type → IL-20RB–/– mice (mean ± SEM clinical score: 0.11 ± 0.02, body weight: 25.99 ± 0.11) showed extreme statistical significance, p < 0.0001 (A). Highest (B) and cumulative scores (C) analysis showed high statistical significance between IL-20RB–/– → wild-type mice (mean ± SEM highest score: 3.44 ± 0.29, cumulative score: 33.19 ± 4.41), and wild-type → IL-20RB–/– mice (mean ± SEM highest score: 0.31 ± 0.13, cumulative score: 2.62 ± 1.37), p < 0.001. Analysis of EAE disease onset for mice with neurologic symptoms (wild-type → wild-type mice and IL-20RB–/– → wild-type mice, 8/8; wild-type → IL-20RB–/– mice, 4/8, and IL-20RB–/– → IL-20RB–/– mice, 5/8) showed statistical significance between IL-20RB–/– → wild-type mice (mean ± SEM: 10.13 ± 0.23) and wild-type → IL-20RB–/– mice (mean ± SEM: 16.50 ± 6.07), p < 0.05 (D). Again, highest and cumulative scores, and disease onset (B–D) of wild-type → wild-type mice (mean ± SEM highest score: 3.38 ± 0.28, cumulative score: 31.88 ± 5.12, disease onset: 11.00 ± 0.50) and IL-20RB–/– → IL-20RB–/– mice (mean ± SEM highest score: 0.44 ± 0.15, cumulative score: 2.88 ± 1.38, disease onset: 16.2 ± 2.33) were similar as the ones observed for wild-type mice and IL-20RB–/– mice with EAE (Figures 1B–D). Analysis of percentage of survival was not deemed significant (E). Results are shown as mean ± SEM, ∗p < 0.05, ∗∗∗p < 0.001, and ****p < 0.0001.