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. 2021 Sep 7;15:683687. doi: 10.3389/fncel.2021.683687

FIGURE 5.

FIGURE 5

IL-20 receptor subunit β expression on rhesus macaque CNS microvessels. CNS region-specific microvessels were isolated from macaque tissue, n = 3, to elucidate IL-20RB protein expression by immunolabeling for VEGFR-2 (white, endothelial marker), IL-20RB (red), AQP4 (green, astrocytic end-feet marker), and DAPI (blue), scale bar = 10 μm (A). VEGFR-2 colocalized with IL-20RB confirming endothelial cells expression of IL-20RB whereas AQP4 appears to envelop both. Histogram analysis was performed to 50 microvessels per specimen and CNS region to determine microvessels diameter and AUI for IL-20RB expression (representative AUI vs. axis plot, B). One-way ANOVA analysis followed by Sidak’s post hoc test showed no statistical significance between AUC means for the different CNS regions (mean ± SD cortex: 6.4 × 10–4 ± 2.9 × 10–5, cerebellum: 6.0 × 10–4 ± 7.3 × 10–5, brainstem: 6.4 × 10–4 ± 2.5 × 10–5 and spinal cord: 7.1 × 10–4 ± 6.7 × 10–5, C). Results are shown as mean ± SD.