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. 2021 Sep 7;12:700501. doi: 10.3389/fimmu.2021.700501

Figure 1.

Figure 1

Monocytes are activated during L. donovani infection in B6 but not in B6.Rag2-/- mice. (A, B) Weight (left panel) and Leishman Donovan Unit (right panel) in the spleen (A) and liver (B) of B6 mice and B6.Rag2-/- at different time point during the course of the infection. Two-way factorial analysis applied to LDUs in (A, B): Spleen P=0.001, Liver P=0.001; statistical power: Spleen 94%, Liver 99%; n≥7; post hoc test EMM: statistical difference reached in spleen and liver at day 56 and day 152 (P<0.0001; ****). (C) Parasite load per one million cells in the bone marrow measured by limiting dilution assay. Two-way factorial analysis applied: P=0.09; statistical power: 32.3%; n≥3; post hoc test EMM: statistical difference reached at day 152 (P<0.0001;****). (D) Gating strategy to identify Ly6C monocytes in BM. (E) Absolute number of iMo cells per femur. Two-way factorial analysis applied: P=0.165; statistical power: 98.5%; n≥7; post hoc test EMM: statistical difference reached at day 28, day 56 and day 152 (*; P=0.014, P=0.036 and P=0.046), respectively. (F, G) MHCII expression on BM Ly6C+CCR2+ cells shown as percentage (F) and as representative flow histogram plots (G) in B6 and B6.Rag2-/- mice during the course of the infection. Two-way factorial analysis applied in (F): P=0.001; statistical power: 99%; n=4; post hoc test EMM: statistical difference reached at day 28, day 56 and 152 (P<0.0001; ****). (H) iNOS and MHCII expression on Ly6C+CCR2+ monocytes. Data are derived from analysis of 8 to 10 individual mice (4 to 5/experiment) of each strain at each time point pooled from two independent experiments and are shown as mean ± SD. Data in (G, H) are representative plots, including lower and upper range of MHCII expression at day 14. In (A–F) median with 95% CI is shown.