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. 2021 Sep 7;12:700501. doi: 10.3389/fimmu.2021.700501

Figure 2.

Figure 2

CD4+ T cells are the main producers of IFNγ in the bone marrow of B6 mice and contribute to local monocyte activation. (A) Dot plot indicating representative expression of IL-10 and IFNγ by CD3+CD4+ T cells at different time points p.i. Cells were stimulated with PMA/Ionomycin. (B) Percentage of CD4+ T cells expressing IFNγ. Welch’s t test applied: P<0.0001 (****); 95% CI: 38.89 – 51.63; statistical power: 100%; n≥4. (C) IFNγ expression by BM CD4+ T cells and MHCII expression on Ly6C+CCR2+ monocytes. Data was normalized by subtraction of d0 mean after arcsin conversion, respectively. (D) Protocol for adoptive transfer of T cells. (E) Cytokine production by adoptively transferred BM CD4+ or CD8+ T cells 1 week post transfer into recipient L. donovani-infected B6.Rag2-/- mice. Cells were stimulated with PMA/Ionomycin. (F, G) MHCII expression on Ly6C+CCR2+ monocytes in the BM of the mice one week post adoptive T cell transfer, shown as histogram plots (F) and percentage (G). Data are derived from analysis of 3 to 4 individual mice of each strain per group and are shown as mean ± SD. Data are pooled from 2 independent experiment. Brown-Forsythe ANOVA applied in (G) without group B6.wt NA: P<0.0001 (****); statistical power: 100%; n≥4; post hoc test Dunnett’s T3, the different pairwise comparisons are shown in Table S1. Data in (E, F) are representative plots.