Figure 3.

Monocytes respond to IFN-γ produced by CD4⁺ T. (A–E) At day 14 p.i, B6.wt mice were injected with an anti-CD4 depleting antibody or a control antibody for 14 days. All the data shown correspond to BM results. (A) Ex vivo IFNγ produced in the BM. Mann-Whitney test applied: P=0.0079 (**); 95% CI: -8.496 – -2.435; statistical power: 96.3%; n=5. Histogram and bar graph representing the expression (B) and percentage (C) respectively, of MHCII on Ly6C⁺CCR2⁺ monocytes. Welch’s t test applied in (C): P=0.0027 (**); 95% CI: -26.26 – -8.97; statistical power: 99.2%; n=5. (D) MFI of MHCII and CD80 on the Ly6C⁺CCR2⁺ monocytes. Welch’s t test applied in (D): P=0.0036 (**); 95% CI: -1053 – -290.5; statistical power: 99.2%; n=5. (E) Parasite burden measured in infected B6 at d28 by limiting dilution assay. Welch’s t test applied in (E): P=0.0082 (**); 95% CI: 0.275 – 1.346; statistical power: 86.3%; n=5. (F–H) Naïve lethally irradiated B6.CD45.1 recipient mice received a 50:50 of BM cells from B6.CD45.1 and B6.Ifngr1^-/- CD45.2 mice. Subsequently infected with 3x10⁷ L. donovani amastigotes for 28 days. (F, G) At day 28, the level of activation of Ly6C⁺CCR2⁺ monocytes cells was determined based on the level of expression of MHCII. Mann-Whitney test applied in G: P=0.0079 (**); 95% CI: -12838 – -8928; statistical power: 100%; n=5. Data are the pool of 5 mice/group/experiment. Mean +/- SD is shown. (H) Dot plot representative of the production of NOS2 and TNF by CD45.1 or CD45.2 Ly6C⁺CCR2⁺ monocytes in the BM.