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. 2021 Sep 7;11:703259. doi: 10.3389/fcimb.2021.703259

Figure 4.

Figure 4

Selected mRNAs with significant changes and overexpression of CACNG4 in EBV-HPV-associated CIN lesions. Expression levels of the selected, significantly altered mRNAs of the mRNA-seq analysis (A) and RT-PCR validation (B). Each value was normalized to the GAPDH value and is presented as the mean ± SD, *p<0.05, compared with the HPV group. (C) A red fluorescence signal indicating the presence of CACNG4 was detected in cervical tissues. (D) Protein expression of CACNG4 relative to β-actin expression in cervical tissues. (E) Densitometric analysis of the immunoblots. The intensity of each band was normalized to the β-actin intensity. Due to the limited amount of clinical material, the EBV-HPV sample consisted of 4 pooled biological replicates and the HPV sample consisted of 3 pooled biological replicates in (D, E). (F) The expression of CACNG4 between cervical cancer samples and normal tissue samples taken from TCGA and the GTEx databases. TPM, transcripts per million.