. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: Brain Behav Immun. 2021 Jun 23;97:42–60. doi: 10.1016/j.bbi.2021.06.011

Table 3.

Statistical values for analyses performed within Figure 3. Mechanical sensitivity and cold allodynia behavioral data were analyzed with a repeated-measures Two-Way ANOVA with Tukey’s post hoc. Effect size datasets were analyzed using Ordinary Two-Way ANOVA with Tukey’s post hoc. Statistical significance was set at p<0.05. All values deemed statistically significant are bolded.

Dataset	Main Effect			Interactions		Multiple Comparisons
		F (DFn, DFd)	p-value	F (DFn, DFd)	p-value	Effect	Groups	POD	p-value
Mechanical Sensitivity	Males	Genotype: F (2, 24) = 89.06 Time: F (3.068, 73.64) = 38.10	p<0.0001 p<0.0001	F (8, 96) = 9.794	p<0.0001	Surgery Genotype	Mixed Sham vs TLR4^fl/fl Mixed Sham vs Na_v1.8^TLR4LoxTB TLR4^LoxTB vs Na_v1.8^TLR4LoxTB	BL 1 3 5 7 BL 1 3 5 7 BL 1 3 5 7	p=0.3123 p=0.0013 p<0.0001 p<0.0001 p<0.0001 p=0.2072 p=0.0036 p<0.0001 p<0.0001 p<0.0001 p=0.7957 p=0.7581 p=0.2611 p=0.4406 p=0.2021
	Females	Genotype: F (2, 24) = 69.27 Time: F (3.278, 78.67) = 19.64	p<0.0001	F (8, 96) = 9.249	p<0.0001	Surgery Genotype	Mixed Sham vs TLR4^fl/fl Mixed Sham vs Na_v1.8^TLR4LoxTB TLR4^LoxTB vs Na_v1.8^TLR4LoxTB	BL 1 3 5 7 BL 1 3 5 7 BL 1 3 5 7	p=0.9777 p=0.0012 p<0.0001 p<0.0001 p<0.0001 p=0.9158 p=0.0266 p=0.0783 p=0.0054 p<0.0001 p=0.7957 p=0.0419 p=0.0074 p=0.0010 p=0.1120
	Effect Size	Genotype: F (2, 49) = 44.27 Sex: F (1, 49) = 0.8985	p<0.0001 p=0.3478	F (2, 49) = 3.384	p=0.0420	Genotype	Male SNI Female SNI		p=0.5528 p=0.0007
Cold Allodynia	Males	Genotype: F (2, 20) = 24.29 Time: F (3.155, 63.10) = 57.32	p<0.0001 p<0.0001	F (8, 80) = 16.68	p<0.0001	Surgery Genotype	Mixed Sham vs TLR4^fl/fl Mixed Sham vs Na_v1.8^TLR4LoxTB TLR4^LoxTB vs Na_v1.8^TLR4LoxTB	BL 1 3 5 7 BL 1 3 5 7 BL 1 3 5 7	p=0.9239 p=0.8930 p=0.0100 p=0.0013 p<0.0001 p=0.7419 p=0.2450 p=0.0010 p=0.0340 p=0.0005 p=0.9249 p=0.3318 p=0.4565 p=0.0850 p=0.0260
	Females	Genotype: F (2, 20) = 21.04 Time: F (2.406, 48.12) = 10.32	p<0.0001 p<0.0001	F (8, 80) = 4.242	p=0.0003	Surgery Genotype	Mixed Sham vs TLR4^fl/fl Mixed Sham vs Na_v1.8^TLR4LoxTB TLR4^LoxTB vs Na_v1.8^TLR4LoxTB	BL 1 3 5 7 BL 1 3 5 7 BL 1 3 5 7	p>0.9999 p=0.7482 p=0.0029 p=0.0009 p=0.0001 p=0.9749 p=0.1593 p=0.0010 p=0.0325 p=0.0004 p=0.9718 p=0.4756 p=0.1864 p=0.9781 p=0.5727
	Effect Size	Genotype: F (2, 40) = 24.96 Sex: F (1, 40) = 2.021	p<0.0001 p=0.1629	F (2, 40) = 0.1819	p=0.1819	Genotype	Male SNI Female SNI		p=0.0368 p=0.9843