. 2021 Sep 7;9:694889. doi: 10.3389/fcell.2021.694889

TABLE 2.

In vitro maturation of canine oocytes for 72 and 48 h in three different media under high O₂ atmosphere.

Group	Time IVM	No. oocytes	GV	MI	MII	DEG	NN
TCM-199 + FBS	72	73	16 (21.9%)^a	24 (32.8%)^a	3 (4.1%)^a	22 (30.1%)^a,c	8 (10.9%)^a
	48	41	8 (19.5%)^a	21 (51.2%)^b	4 (9.7%)^a	3 (7.3%)^b	5 (12.1%)^a
SOF + BSA + FBS	72	86	17 (19.7%)^a	23 (26.9%)^a	6 (6.9%)^a	20 (23.2%)^c	20 (23.2%)^b
	48	55	16 (29%)^a	27 (49%)^b	2 (3.6%)^a	5 (9%)^b	5 (9%)^a
SOF + BSA	72	78	16 (20.5%)^a	21 (26.9%)^a	4 (5.5%)^a	34 (43.5%)^a	3 (4.1%)^a

The percentages of oocytes in the germinal vesicle (GV), metaphase I (MI), metaphase II (MII), degenerated (DEG), and non-identifiable (NN) stages were recorded. In the same column, percentages without the same superscripts differed significantly (^a−cp-value < 0.05). The experiments were repeated three times (n = 3).

BSA, bovine serum albumin; FBS, fetal bovine serum; IVM, in vitro maturation; SOF, synthetic oviductal fluid.