. 2021 Sep 7;9:694889. doi: 10.3389/fcell.2021.694889

TABLE 3.

The effect of ITS supplementation on canine IVM for 72 h under high O₂ atmosphere.

Group	No. oocytes	GV	MI	MII	DEG	NN
SOF + BSA + FBS	71	16 (22.5%)^a	37 (52.1%)^a	3 (4.2%)^a	7 (9.8%)^a,b	8 (11.2%)^a
SOF + BSA + FBS + 1 μl/ml ITS	81	16 (19.7%)^a	32 (39.5%)^a	16 (19.7%)^b	7 (8.6%)^a	10 (12.3%)^a
SOF + BSA + FBS + 10 μl/ml ITS	80	16 (20%)^a	29 (36.2%)^a	5 (6.2%)^a	17 (21.2%)^b	13 (16.2%)^a

SOF + BSA + FBS (control): COCs cultured in SOF + 8% BSA + 2.5% FBS; SOF + BSA + FBS + 1 μl/ml ITS: COCs cultured in SOF + 8% BSA + 2.5% FBS and 1 μl/ml ITS; and SOF + BSA + FBS + 10 μl/ml ITS: COCs cultured in SOF + 8% BSA + 2.5% FBS and 10 μl/ml ITS. The percentages of oocytes in the germinal vesicle (GV), metaphase I (MI), metaphase II (MII), degenerated (DEG), and non-identifiable (NN) stages was recorded. In the same column, percentages with different superscripts differed significantly (^a,bp < 0.05). The experiments were repeated three times (n = 3).

BSA, bovine serum albumin; COC, cumulus–oocyte complex; FBS, fetal bovine serum; ITS, insulin-transferrin-selenium; IVM, in vitro maturation; SOF, synthetic oviductal fluid.