. 2021 Sep 7;9:694889. doi: 10.3389/fcell.2021.694889

TABLE 4.

In vitro maturation of canine oocytes in SOF + BSA + FBS medium for 48 h under two different O₂ tension atmospheres in the presence or absence of ITS.

Group	No. oocytes	GV	MI	MII	DEG	NN
SOF + BSA + FBS + high O₂	64	26 (40.6%)^a	27 (42.2%)^a	5 (7.8%)^a	6 (9.4%)^a	0 (0%)^a
SOF + BSA + FBS + 1 μl/ml ITS + high O₂	53	16 (30.2%)^a,c	20 (37.7%)^a	13 (24.5%)^b	2 (3.8%)^a	2 (3.8%)^a
SOF + BSA + FBS + low O₂	63	5 (7.9%)^b	32 (50.8%)^a	23 (36.5%)^b	1 (1.6%)^a	2 (3.2%)^a
SOF + BSA + FBS + 1 μl/ml ITS + low O₂	65	11 (16.9%)^b,c	34 (52.3%)^a	17 (26.2%)^b	2 (3.1%)^a	1 (1.5%)^a

The percentages of oocytes in the germinal vesicle (GV), metaphase I (MI), metaphase II (MII), degenerated (DEG), and non-identifiable (NN) stages were recorded. In the same column, percentages with different superscripts differed significantly (^a−bp < 0.05). The experiments were repeated three times (n = 3).

BSA, bovine serum albumin; FBS, fetal bovine serum; ITS, insulin-transferrin-selenium; IVM, in vitro maturation; SOF, synthetic oviductal fluid.