. 2021 Sep 7;9:694889. doi: 10.3389/fcell.2021.694889

TABLE 5.

Viability and IVM of canine oocytes in SOF + BSA + FBS medium for 48 h under low O₂ tension atmospheres in the presence or absence of ITS.

Group	No. oocytes	Viability	GV + MI	MII	NN
SOF + BSA + FBS + low O₂	67	16 (23.8%)^a	59 (88.1%)^a	8 (11.9%)^a	9 (13.4%)^a
SOF + BSA + FBS + 1 μl/ml ITS + low O₂	71	40 (56.3%)^b	53 (74.6%)^a	18 (25.3%)^b	10 (14.1%)^a

Viability and IVM of oocyte after co-staining with propidium iodide (PI) and Hoechst 33342 to determine maturation and cell membrane integrity. The percentages of oocytes in the germinal vesicle or metaphase I (GV + MI), metaphase II (MII), and non-identifiable (NN) stages were recorded simultaneously with cell membrane integrity evaluation after 48 h of IVM. In the same column, percentages with different superscripts differed significantly (^a,bp < 0.05). The experiments were repeated three times (n = 3).

BSA, bovine serum albumin; FBS, fetal bovine serum; IVM, in vitro maturation; ITS, insulin-transferrin-selenium; SOF, synthetic oviductal fluid.