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. 2021 Sep 7;12:636494. doi: 10.3389/fneur.2021.636494

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Copyright © 2021 Li, Guo, Sun, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of DCS on the viability of cultured primary neurons exposed to oxygen-glucose deprivation/reperfusion (OGD/R). (A) Cultured primary neurons isolated from rat E18 embryos and immunostained for neurofilament (NF, expressed in neurons; green) and glial fibrillary acidic protein (GFAP, expressed in astrocytes; red). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Neuronal cell purity exceeded 95%. (B) Cell viability determined by using Cell Counting Kit-8. Primary neurons were exposed to OGD/R and administered DCS for 4 h at various concentrations (0, 0.2, 0.4, 0.6, 0.8, and 1 μg/μl). Cells not exposed to OGD/R were used as a blank control group. Data shown as mean ± SEM, n = 3. *p < 0.05, ***p < 0.0005.