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. 2021 Sep 7;12:636494. doi: 10.3389/fneur.2021.636494

Figure 3.

Figure 3

Effect of DCS on the apoptosis of cultured primary neurons exposed to OGD/R. (A) The apoptosis of cultured primary neurons isolated from rat E18 embryos assessed using a phycoerythrin/Annexin-V assay and flow cytometry. In the FACS picture, the upper-left quadrant represents mechanically damaged cells, the upper-right quadrant apoptotic or necrotic cells, the lower-left quadrant normal cells, and the lower-right quadrant early apoptotic cells. (B) The apoptosis of cultured primary neurons isolated from rat E18 embryos assessed using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Blank control: cells not exposed to OGD/R; OGD/R: cells exposed to OGD/R; OGD/R + 0.4 μg/μl DCS: cells exposed to OGD/R and treated with 0.4 μg/μl of DCS for 4 h. Data shown as mean ± SEM, n = 3. *p < 0.05, ***p < 0.005.