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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Toxicology. 2021 Aug 17;461:152900. doi: 10.1016/j.tox.2021.152900

Table 1.

Descriptive Statistics for Laboratory-Specific Differentiation Protocols

Laboratory cells/laboratory protocol
Participating
Laboratory		 Cell
Source		 Cell Lot # Passage Test
Chem
Length
(d)		 Diff.
Length
(d)		 Cocktail
Length
(d)		 TC
Plate		 TC
plate
#		 Base
Media		 IB
MX		 Insulin Dexametha
sone		 Biotin Ca
Pantothenate		 FBS
Lab 1 ATCC Batch 6 u+3 10 10 2 Falcon 353219 DMEM-LG 0.5 mM 0.6 mg/mL 250 nM - - Wisent #090150, Lot#115680
Lab 2 ATCC 700009858 u+3, 9 10 10 2 Greiner BioOne 82050-748 DMEM-HG 0.5 mM 1 mg/mL 250 nM - - Euroclone, ECS0180L, lot # EUS0040912
Lab 3 ATCC 63343749 u+3 10 10 2 Corning 07-200-565 DMEM-HG 0.5 mM 1 mg/mL 250 nM - - Sigma F6178, Lot #013K8411
Lab 4 ATCC MBXclone1 u+5 10 10 2 Greiner BioOne 82050-748 DMEM/F12 0.5 mM 1 mg/mL - 33 mM 17 mM Fisher Scientific (Gibco) 10437-028
Lab 5 ATCC 2268173 u+9 14 14 2 Greiner BioOne 82050-748 DMEM-HG 0.5 mM 1 mg/mL 20 nM - - Sigma #F2442, lot 1982183
Lab 6 ATCC - u+3, 8 10 10 2 Greiner BioOne 82050-748 DMEM-HG 0.5 mM 10 mg/mL 1 mM - - GE Healthcare life sciences, SH30910.03HI
Lab 7 ATCC 63891946 u+3 10 10 2 Greiner CellStar 655090 DMEM-HG 0.5 mM 10 mg/mL - - - GIBCO; Ref: 10270-106; Lot: 41G1780K
Lab 8 Green2 - 11 8 8 2 Falcon 353219 DMEM-HG 125 mM 2.5 ug/mL 1.25 mM 2 mg/L 1 mg/L Gemini #100-106
Lab 9 Zenbio 3T3L1062104 10 10 10 2 Greiner CellStar M0562-32EA DMEM-HG 0.5 mM 1 mg/mL - - - Sigma F9665
Lab 10 ATCC - u+4 11 11 2 USA Scientific 5665-5087 DMEM-HG 0.5 mM 10 mg/mL 1 mM - - Hyclone SH30541.03
Shared Zenbio 3T3L1062104 8 10 10 2 Greiner BioOne 82050-748 DMEM-HG 0.5 mM 1 mg/mL - - -

Descriptive statistics for laboratory-specific differentiation protocols provided by each participating laboratory. These cells and differentiation details were utilized for the LC and LP conditions only. The “Shared” cell source was utilized for all SC conditions, and the shared protocol (Supplemental information) for all SP conditions. All laboratories completed tests using each of the four defined test conditions.

1

3T3-L1 MBX clone utilized for these experiments: designed to ensure more complete adipocyte differentiation and insulin sensitivity; unknown lot #

2

Green H (isolating laboratory) source as gift from Philip Pekala (same apparent source as commercial Zenbio cells); no reported lot #

u = unknown passage number

Differentiation length specifies the total duration of adipocyte differentiation and adipocyte maintenance media treatment, whereas cocktail length specifies just the duration of the differentiation induction media treatment.