Genetic deletion of LCN2 improves nest building during cancer cachexia, but not LPS challenge. (a) Experimental design for pancreatic tumor and LPS studies. (b) Nest building scores in male Lcn2-KO and WT mice after sham operation or tumor implantation (Average of 2 consecutive scoring days; n = 6–12 per group; WT sham vs. tumor, p=0.0267). (c) Nest building scores in Lcn2-KO and WT mice 24 hours after LPS treatment (n = 5–7 per group; Lcn2-KO vehicle vs LPS, p=0.0259). Data in b-c are expressed as mean ± SEM. Data represented in b-c were analyzed with two-way ANOVA with Bonferroni multiple comparisons per cell type. LPS dose was 1 mg/kg. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001, ****p ≤ 0.0001. LPS = lipopolysaccharide; IP = intraperitoneal.