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. 2021 Sep 7;12:736498. doi: 10.3389/fimmu.2021.736498

Figure 2.

Figure 2

IDO1 is expressed by immune cells in the lymph node and correlates with altered frequencies of circulating monocytes and lymphocytes. (A) Representative immunohistochemical images of IDO1 expression in the paracortex and (B) in the sinuses (indicated by arrows) of the lymph node. (C) (top) Gating strategy of circulating monocytes and lymphocytes in viable PBMCs. (middle) Boxplots with monocyte-to-lymphocyte ratio (MLR), frequency of monocytes and lymphocytes according to IDO1 expression in the paracortex and (bottom) IDO1 expression in the sinuses. (D) Boxplots with the concentration of indicated cytokine in supernatants of anti-CD3/CD28 activated PBMCs according to IDO1 expression in the sinuses. The cytokine concentration was divided by the percentage of viable circulating lymphocytes per patient. (C, D) P value calculated using two-sided Mann-Whitney U test. *p < 0.05, **p < 0.01. Whiskers of boxplots extend to the minimum and maximum data point, with the horizontal line indicating the median.