Table 1.
Genotypic features of the Streptomyces and Escherichia coli strains used in this study.
| Strain | Features | Source |
|---|---|---|
| S. albus J1074 (C416-M)-OE3 | Based on S. albus J1074 (C4I6-M) in which a BAC plasmid containing the spinosad biosynthetic gene cluster is integrated into the chromosome of S. albus J1074, an extra spnI gene was overexpressed under the control of rpsLp-cf, gtt and epi were co-overexpressed under the control of kasOp*, gdh and kre were co-overexpressed under the control of kasOp*, spnO and spnN were co-overexpressed under the control of kasOp*, spnQ and spnR were co-overexpressed under the control of rpsLp-cf, spnS was overexpressed under the control of rpsLp-tp, and the PKS gene spnE was overexpressed by kasOp*. | Tan et al., 2017 [30] |
| S. coelicolor CH999 | proA1, argA1 redE60 Δact::ermE SCP−, SCP2− | McDaniel et al., [43] |
| AE | The PKS operon spnABC was overexpressed under the control of the rpsLp-cf promoter in S. albus J1074 (C416-M)-OE3 | This study |
| ADE | The PKS gene spnD was overexpressed under the control of the rpsLp-tp promoter in AE | This study |
| ACDE | The PKS gene spnC was individually overexpressed under the control of the kasOp* promoter in ADE | This study |
| ABDE | The PKS operon spnBC was overexpressed under the control of the kasOp*- rpsLp-cf promoter in ADE | This study |
| ABCDE | The PKS genes spnB and spnC were overexpressed under the control of kasOp*-rpsLp-cf, and kasOp* promoters in ADE, respectively | This study |
| ABE | The PKS operon spnBC was overexpressed under the control of the kasOp*- rpsLp-cf promoter in AE | This study |
| DcanP2 | Deletion part of candicidin PKS gene in ADE | This study |
| ADE-AP | Extra acsA and pccBE were overexpressed under the control of kasOp*, additional accA2 and accBE were overexpressed under the control of rpsLp-cf in ADE, PKS of candicidin BGC in ADE was replaced by acsA and pccBE to interrupt the expression of the candicidin PKS gene | This study |
| E. coli DH10b | F− mcrA Δ(mrr-hsdRMS-mcrBC) | Gibco-BRL |
| E. coli ET12567(pUB307) | dam dcm hsdS/pUB307 | Flett et al., 1997 [44] |