Table 1.
Minocycline-Related Effects on the Expression of Inflammatory and Oxidative Markers in VH and MIS Offspring
| Inflammatory/oxidative markers | MIS | Treatment | Interaction | |||
|---|---|---|---|---|---|---|
| iNOS | ||||||
| Prefrontal cortex | F(1,28) = 9.80** | η 2p = 0.26 | F(1,28) = 4.090.053 | η 2p = 0.13 | F(1,28) = 17.52*** | η 2p = 0.39 |
| Hippocampus | F(1,28) = 0.75 | η 2p = 0.03 | F(1,28) = 1.60 | η 2p = 0.05 | F(1,28) = 0.44 | η 2p = 0.01 |
| Amygdala | F(1,28) = 13.55*** | η 2p = 0.33 | F(1,28) = 0.25 | η 2p = 0.01 | F(1,28) = 3.52 | η 2p = 0.11 |
| Caudate-putamen | F(1,28) = 7.72** | η 2p = 0.22 | F(1,28) = 8.87** | η 2p = 0.24 | F(1,28) = 4.75* | η 2p = 0.14 |
| COX2 | ||||||
| Prefrontal cortex | F(1,28) = 0.02 | η 2p < 0.01 | F(1,28) = 0.57 | η 2p = 0.20 | F(1,28) = 0.02 | η 2p < 0.01 |
| Hippocampus | F(1,28) = 1.49 | η 2p = 0.05 | F(1,28) = 0.02 | η 2p < 0.01 | F(1,28) < 0.001 | η 2p < 0.01 |
| Amygdala | F(1,28) = 8.94** | η 2p = 0.24 | F(1,28) = 7.42* | η 2p = 0.21 | F(1,28) = 1.40 | η 2p = 0.05 |
| Caudate-putamen | F(1,28) = 1.06 | η 2p = 0.04 | F(1,28) = 2.22 | η 2p = 0.07 | F(1,28) = 0.09 | η 2p < 0.01 |
| Oxidative markers | ||||||
| KEAP1 | ||||||
| Prefrontal cortex | F(1,27) = 20.08*** | η 2p = 0.43 | F(1,27) = 0.54 | η 2p = 0.20 | F(1,27) = 0.07 | η 2p < 0.01 |
| Hippocampus | F(1,28) = 0.05 | η 2p < 0.01 | F(1,28) = 13.49** | η 2p = 0.33 | F(1,28) = 3.76 | η 2p = 0.12 |
| Amygdala | F(1,28) = 28.63*** | η 2p = 0.51 | F(1,28) = 3.52 | η 2p = 0.11 | F(1,28) = 0.22 | η 2p < 0.01 |
| Caudate-putamen | F(1,28) = 5.42* | η 2p = 0.16 | F(1,28) < 0.001 | η 2p < 0.01 | F(1,28) = 0.33 | η 2p = 0.01 |
| NRF2 | ||||||
| Prefrontal cortex | F(1,27) = 0.14 | η 2p < 0.01 | F(1,27) = 0.01 | η 2p < 0.01 | F(1,27) = 3.41 | η 2p = 0.11 |
| Hippocampus | F(1,26) = 0.53 | η 2p = 0.02 | F(1,26) = 0.05 | η 2p < 0.01 | F(1,26) = 1.70 | η 2p = 0.06 |
| Amygdala | F(1,28) = 0.13 | η 2p < 0.01 | F(1,28) = 0.51 | η 2p = 0.02 | F(1,28) = 0.15 | η 2p < 0.01 |
| Caudate-putamen | F(1,26) = 0.53 | η 2p < 0.01 | F(1,26) = 0.05 | η 2p < 0.01 | F(1,26) = 1.70 | η 2p < 0.01 |
| HO1 | ||||||
| Prefrontal cortex | F(1,28) = 0.69 | η 2p = 0.02 | F(1,28) = 7.81** | η 2p = 0.22 | F(1,28) = 4.93* | η 2p = 0.15 |
| Hippocampus | F(1,28) = 0.03 | η 2p < 0.01 | F(1,28) = 2.00 | η 2p = 0.07 | F(1,28) = 1.99 | η 2p = 0.07 |
| Amygdala | F(1,28) = 17.60*** | η 2p = 0.39 | F(1,28) = 25.28*** | η 2p = 0.48 | F(1,28) = 9.60** | η 2p = 0.26 |
| Caudate-putamen | F(1,28) = 1.12 | η 2p = 0.04 | F(1,28) = 5.66* | η 2p = 0.17 | F(1,28) = 1.42 | η 2p = 0.05 |
| NQO1 | ||||||
| Prefrontal cortex | F(1,28) = 4.110.052 | η 2p = 0.13 | F(1,28) = 0.14 | η 2p < 0.01 | F(1,28) = 1.03 | η 2p = 0.36 |
| Hippocampus | F(1,28) = 0.38 | η 2p = 0.01 | F(1,28) = 13.80*** | η 2p = 0.33 | F(1,28) = 1.78 | η 2p = 0.06 |
| Amygdala | F(1,28) = 0.28 | η 2p = 0.01 | F(1,28) = 3.16 | η 2p = 0.10 | F(1,28) = 3.54 | η 2p = 0.11 |
| Caudate-putamen | F(1,28) = 0.28 | η 2p = 0.01 | F(1,28) = 0.8 | η 2p = 0.01 | F(1,28) = 0.02 | η 2p < 0.01 |
| SOD | ||||||
| Prefrontal cortex | F(1,28) = 0.42 | η 2p = 0.02 | F(1,28) = 0.01 | η 2p < 0.01 | F(1,28) = 0.25 | η 2p = 0.01 |
| Hippocampus | F(1,27) = 5.19* | η 2p = 0.16 | F(1,27) = 0.38 | η 2p = 0.01 | F(1,27) = 0.59 | η 2p = 0.02 |
| Amygdala | F(1,28) = 0.01 | η 2p < 0.01 | F(1,28) = 2.72 | η 2p = 0.09 | F(1,28) = 5.84* | η 2p = 0.17 |
| Caudate-putamen | F(1,28) = 0.01 | η 2p < 0.01 | F(1,28) = 1.35 | η 2p = 0.05 | F(1,28) = 5.84* | η 2p = 0.17 |
| CAT | ||||||
| Prefrontal cortex | F(1,28) = 2.12 | η 2p = 0.07 | F(1,28) = 2.25 | η 2p = 0.07 | F(1,28) = 0.59 | η 2p = 0.02 |
| Hippocampus | F(1,28) = 0.001 | η 2p < 0.01 | F(1,28) = 0.41 | η 2p = 0.01 | F(1,28) = 2.78 | η 2p = 0.10 |
| Amygdala | F(1,28) = 0.54 | η 2p = 0.02 | F(1,28) = 0.04 | η 2p < 0.01 | F(1,28) = 2.08 | η 2p = 0.07 |
| Caudate-putamen | F(1,27) = 4.97* | η 2p = 0.18 | F(1,27) = 2.53 | η 2p = 0.04 | F(1,27) = 0.06 | η 2p = 0.01 |
| GSHfree | ||||||
| Prefrontal cortex | F(1,28) = 3.23 | η 2p = 0.10 | F(1,28) = 2.34 | η 2p = 0.08 | F(1,28) = 0.01 | η 2p < 0.01 |
| Hippocampus | F(1,28) = 1.72 | η 2p = 0.06 | F(1,28) = 3.50 | η 2p = 0.11 | F(1,28) = 1.01 | η 2p = 0.03 |
| Amygdala | F(1,28) = 0.94 | η 2p = 0.03 | F(1,28) = 0.11 | η 2p < 0.01 | F(1,28) = 5.64* | η 2p = 0.17 |
| Caudate-putamen | F(1,28) = 0.81 | η 2p = 0.03 | F(1,28) = 0.26 | η 2p = 0.01 | F(1,28) = 3.40 | η 2p = 0.11 |
| GSHtotal | ||||||
| Prefrontal cortex | F(1,28) = 3.41 | η 2p = 0.11 | F(1,28) = 2.59 | η 2p = 0.09 | F(1,28) = 0.003 | η 2p < 0.01 |
| Hippocampus | F(1,28) = 1.43 | η 2p = 0.05 | F(1,28) = 7.80** | η 2p = 0.22 | F(1,28) = 1.59 | η 2p = 0.05 |
| Amygdala | F(1,28) = 0.48 | η 2p = 0.02 | F(1,28) = 1.04 | η 2p = 0.04 | F(1,28) = 6.10* | η 2p = 0.18 |
| Caudate-putamen | F(1,26) = 2.16 | η 2p = 0.08 | F(1,26) = 0.98 | η 2p = 0.04 | F(1,26) = 1.85 | η 2p = 0.07 |
| GSHfree/GSSG | ||||||
| Prefrontal cortex | F(1,28) = 0.002 | η 2p < 0.01 | F(1,28) = 0.05 | η 2p < 0.01 | F(1,28) = 1.27 | η 2p = 0.04 |
| Hippocampus | F(1,28) = 2.07 | η 2p = 0.07 | F(1,28) = 6.47* | η 2p = 0.18 | F(1,28) = 0.12 | η 2p < 0.01 |
| Amygdala | F(1,27) = 1.91 | η 2p = 0.07 | F(1,27) = 0.83 | η 2p = 0.03 | F(1,27) = 3.09 | η 2p = 0.10 |
| Caudate-putamen | F(1,28) = 2.80 | η 2p = 0.09 | F(1,28) = 0.04 | η 2p < 0.01 | F(1,28) = 1.76 | η 2p = 0.06 |
| GPx | ||||||
| Prefrontal cortex | F(1,27) = 0.03 | η 2p < 0.01 | F(1,27) = 0.14 | η 2p < 0.01 | F(1,27) = 4.43* | η 2p = 0.14 |
| Hippocampus | F(1,27) = 0.74 | η 2p = 0.03 | F(1,27) = 7.35* | η 2p = 0.21 | F(1,27) = 0.43 | η 2p = 0.02 |
| Amygdala | F(1,28) = 0.08 | η 2p < 0.01 | F(1,28)<0.001 | η 2p < 0.01 | F(1,28) = 1.38 | η 2p = 0.05 |
| Caudate-putamen | F(1,28) = 1.52 | η 2p = 0.05 | F(1,28) = 0.04 | η 2p < 0.01 | F(1,28) = 4.010.054 | η 2p = 0.13 |
| MDA | ||||||
| Prefrontal cortex | F(1,27) = 6.56* | η 2p = 0.25 | F(1, 27) = 0.53 | η 2p = 0.06 | F(1,27) = 0.53 | η 2p < 0.01 |
| Hippocampus | F(1,27) = 2.03 | η 2p = 0.70 | F(1,27)<0.001 | η 2p < 0.01 | F(1,27) = 1.62 | η 2p = 0.06 |
| Amygdala | F(1,28) = 0.46 | η 2p = 0.02 | F(1,28) = 0.49 | η 2p = 0.02 | F(1,28) = 0.03 | η 2p < 0.01 |
| Caudate-putamen | F(1,27) = 1.10 | η 2p = 0.04 | F(1,27) = 0.39 | η 2p = 0.01 | F(1,27) = 0.14 | η 2p < 0.01 |
Abbreviations: CAT, catalase; COX2, cyclooxygenase-2; F, ANOVA F-test (*P < .05, **P < .01, ***P < .001); GSH, glutathione; GSSG, oxidized glutathione; GPx, glutathione peroxidase; HO1, heme oxygenase 1; iNOS: inducible nitric oxide synthase; KEAP1, Kelch like ECH associated protein 1; MDA, malondialdehyde; NRF2, nuclear factor-erythroid factor 2-related factor 2; NQO1, NAD(P)H:quinone oxidoreductase-1; SOD, superoxide dismutase; η 2p, partial eta-squared. Each column represents the ANOVA F-test and η 2p for MIS, minocycline treatment, and its interaction for the studied areas.