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. 2019 Oct 7;116(10):1721–1732. doi: 10.1093/cvr/cvz262

Figure 3.

Figure 3

TLR4 signalling is involved in flow pattern-mediated PCSK9 expression. (A) TLR4 inhibitor TAK-242 and its specific inhibitor peptide decrease PCSK9 expression at both protein (upper panel) and mRNA level (lower panel). (B) MyD88 inhibitor Pepinh-MYD and its specific inhibitor peptide decrease protein and mRNA expression of PCSK9. (C) TRIF inhibitor Pepinh-TRIF and its monoclonal antibody almost have no effect on PCSK9 expression at both protein and mRNA level (lower panel). (D) NF-κB inhibitors BaY11-7085 and Helenalin decrease PCSK9 expression. The rabbit thoracic aorta with length points between 0 and 0.6 cm were used in (A) to (D), treatment with LPS at 20 ng/mL in the perfusion medium for 12 h. Bar graphs represent data compiled from three independent experiments (n = 5 rabbit per genotype), shown as mean ± standard deviation. The significances between two groups were tested by unpaired t-test; Multiple comparisons were analysed by one-way ANOVA, followed by Tukey’s post hoc comparisons test (*P < 0.05, **P < 0.01; ****P < 0.0001).