TABLE 5.
A constitutively active form of Gpa2p interacts with Ime2p in the two-hybrid assaya
| Protein(s) | β-Galactosidase activityb
|
|
|---|---|---|
| Color on plate | U (10) in liquid medium (mean ± SD) | |
| Fusion proteins | ||
| BD-Gpa2p + AD-Gal4p | White | 4.5 ± 1.3 |
| BD-Gpa2pG132V + AD-Ime2CT | Blue | 469 ± 100 |
| BD-Gpa2pG132V + AD-Ime2p | Blue | 21 ± 4 |
| BD-Gpa2pG299A + AD-Ime2CT | Blue | 463 ± 105 |
| BD-Gpa2pG299A + AD-Ime2p | White | 5.9 ± 1.5 |
| Gal4p wild type | Blue | 1260 ± 200 |
a
Strain Y190 was transformed with pACT2-IME2CT or pACT2-IME2 and crossed with strain Y187 transformed with either pAS2-GPA2/G132V or pAS2-GPA2/G299A.
b
Tested both on filter and in liquid medium, using protein extracts (11).