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. 2021 Sep 6;22(5):1260. doi: 10.3892/etm.2021.10695

Figure 2.

Figure 2

XIST acts as a sponge for miR-485. (A) Potential binding site of miR-485 with the XIST sequence. (B) miR-485 mimic upregulated the expression of miR-485 in RAFLSs. (C) Luciferase activity reporter assays demonstrated that the miR-485 mimic inhibited luciferase activity in the XIST wild-type. (D) TNF-α could downregulate the level of miR-485 expression in RAFLSs, which was rescued by quercetin. (E) The expression of XIST was significantly downregulated in RAFLSs transfected with si-XIST. (F) XIST-silencing effectively promoted the expression of miR-485, which was abolished by miR-485 inhibitor in RAFLSs treated with TNF-α. *P<0.05 vs. NC group. XIST, X-inactive specific transcript; miR/miRNA, microRNA; NC, negative control; si-, short interfering; mut; mutant; RAFLSs, RA fibroblast-like synoviocytes.