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. 2021 Sep 6;22(5):1260. doi: 10.3892/etm.2021.10695

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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PSMB8 is a direct target of miR-485. (A) A highly conserved putative binding site with miR-485 was identified at the PSMB8 3'-untranslated region. (B) miR-485 mimic could repress the fluorescence in the PSMB8 wild-type. (C) TNF-α could significantly upregulate the level of PSMB8 expression in RAFLSs, which was reversed by quercetin. (D) miR-485 inhibitor could downregulate the expression of miR-485 in RAFLSs. (E) Expression of PSMB8 was significantly decreased in RAFLSs after transfection with si-PSMB8. (F) miR-485 inhibitor could significantly increase PSMB8 expression at the mRNA and protein levels, which was abolished by si-PSMB8 in RAFLSs treated with TNF-α. ^*P<0.05 vs. NC group. PSMB8, proteasome subunit β type-8; miR/miRNA, microRNA; si-, short interfering; NC, negative control; RAFLSs, rheumatoid arthritis fibroblast-like synoviocytes.