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. 2021 Aug 26;22(5):1222. doi: 10.3892/etm.2021.10656

Figure 3.

Figure 3

RvD2 promotes NLRP3 degradation through autophagy. (A-C) IL-1β level in the supernatant of macrophage pretreated for 1 h with the autophagy inhibitors 3-MA and bafilomycin A, the proteasome inhibitor MG-132, and stimulated with LPS and Nig. RvD2 (10 nM) was added for 30 min before LPS stimulation (n=3). (D and E) Western blot analysis and (F-I) quantification of (F and G) NLRP3, (H and I) LC3 and β-actin in cell lysate in the presence of (D,. F,. G) 3-MA or (E, H, I) MG-132 (n=3). *P<0.05. 3-MA, 3-methyladenine; IL, interleukin; LC3, microtubule-associated proteins 1A/1B light chain 3; LPS, lipopolysaccharide; Nig, nigericin; NLRP3, NLR family pyrin domain containing 3; NS, non-significant; RvD2, resolvin D2.