FIGURE 5.

Wet mounts from ovarian biopsies from different greater amberjack Seriola dumerili breeders at the onset of the spawning season, showing (a) oocytes at the very early stage of follicular atresia (eFA), and (b) oocytes undergoing early maturation (eOM). Vitellogenic oocytes (vg), as well as primary oocytes (po) and cortical alveoli oocytes (ca) may also exist in the biopsies. Histological sections from the same ovarian biopsies show vitellogenic and eFA oocytes (c and e), the latter exhibiting zona radiata (zr) enlargement and fragmentation, and ooplasm disorganization; and eOM oocytes (d and f) showing lipid droplet (arrows) coalescence into eventually a single oil droplet (od), while the germinal vesicle (nucleus, n) is still intact and the yolk globules still dispersed. Magnification bars =500 μm (a, d) and 200 μm (e, f)