Figure 5.

ML-7 treated mouse eggs have a reduced zinc spark upon ICSI. (A) Representative time traces of the zinc spark (F/F₀) and calcium transients of mouse eggs upon fertilisation via ICSI, of ML-7 treated or control eggs. Red arrows signify the injection of sperm. (B) Dot graphs quantifying the amplitudes of the intensity of calcium and zinc sparks after ICSI between ML-7-treated (N = 5) and control groups (N = 8). ML-7-treated eggs had a 25% reduced zinc spark amplitude after fertilisation via ICSI compared with controls. Data were presented as the mean (mean ± standard error or the mean). (^*  P < 0.05).