TABLE 2.
Histological and Immunohistochemical Features of Subjects That Had a Diagnosis of PXA Both by Histology and by DNA Methylation
| Subject# | Sex | Age (Years) | Classification Score | Tumor Location | Anaplastic Features | Eosinophilic Granular Bodies | Pericellular Reticulin | Necrosis | Mitoses | Microvascular Proliferation | Ki-67 Index | Immunohistochemistry | BRAF V600E Status |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Female | 17 | 0.99 | Right temporal lobe | No | Yes | N/A | No | 4/10 HPF | No | 7% | GFAP+, p53+ | Positive |
| 2 | Male | 18 | 0.99 | Left parasagittal frontal lobe | No | No | None | No | Rare | No | 5% | GFAP+ Synaptophysin+ | Positive |
| 3 | Male | 23 | 0.93 | Right parietal lobe | Yes | Yes | None | Yes | >5/10 HPF | Yes | 1–2%, focally 7.1% | GFAP+, S100+ | Positive |
| 4 | Male | 10 | 0.99 | Right temporal lobe | Yes | Yes | N/A | No | 7/10 HPF | No | “Elevated” | GFAP+, Synaptophysin+, p53+ | Negative |
| 5 | Female | 16 | 0.99 | Left frontal lobe | Yes | Yes | N/A | Yes | N/A | Yes | “Elevated” | GFAP+, Synaptophysin+ | Positive |
| 6 | Female | 10 | 0.99 | Left temporal lobe | Yes | Yes | None | No | 5/10 HPF | No | N/A | GFAP+, p53+ | Negative |
| 7 | Female | 32 | 0.99 | Right temporal parietal mass | Yes | Yes | N/A | No | 6/10 HPF | No | 10% | GFAP+, Synaptophysin– | Positive |
| 8 | Male | 25 | 0.99 | Left temporal region | No | No | N/A | No | None | No | 4% | GFAP+, Synaptophysin+, p53– | Positive |
| 9 | Male | 8 | 0.99 | Right temporal lobe | No | Yes | N/A | No | Rare | No | 10–15% | GFAP+, Synaptophysin– | Positive |
| 10 | Female | 23 | 0.99 | Right parietal lobe | Yes | Yes | N/A | Yes | <5/10 HPF | No | 15% | GFAP+, Synaptophysin+, p53+ | Positive |
| 11 | Male | 11 | 0.99 | Left frontoparietal | Yes | No | Focally+ | No | 10/10 HPF | No | 40–50% | GFAP+, Synaptophysin+, S100+, p53+ | Positive |
| 12 | Male | 15 | 0.88 | Left frontal lobe | Yes | Yes | None | Yes | 5/10 HPF | Yes | 1–3%, focally 5–10% | GFAP+, Synaptophysin+ | Positive |
| 13 | Male | 16 | 0.62 | Right temporal lobe | Yes | Yes | Diffusely+ | No | >5/10 HPF | No | 5–10% | GFAP+, S100+, p53+ | N/A |
| 14 | Female | 16 | 0.99 | Right Parietal/superficial | Yes | Yes | Diffusely+ | No | >5/10 HPF | No | 5–20% | GFAP+, p53+ | N/A |
| 15 | Female | 9 | 0.99 | Right frontal lobe | Yes | Yes | Diffusely+ | No | >5/10 HPF | No | 10–15% | GFAP+, S100+, p53+ | N/A |
| 16 | Male | 7 | 0.91 | Left frontal lobe | Yes | Yes | Focally+ | Yes | >5/10 HPF | No | up to 20% | GFAP+, Synaptophysin+, p53+ | Positive |
| 17 | Female | 10 | 0.99 | Left parietal lobe | No | Yes | Diffusely+ | No | 3/10 HPF | No | <5% | GFAP+, S100+, p53 ≤ 5% | N/A |
| 18 | Female | 7 | 0.99 | Right parietal lobe | No | Yes | Patchy+ | No | <1/10 HPF | No | <5% | GFAP+, Synaptophysin+, p53– | N/A |