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Schematic overview of disulfide rebridging: (a) A protein (blue) containing a disulfide (1) is reduced to thiols (2) by tris(2‐carboxyethyl)phosphine (TCEP), followed by a reaction with a bis‐electrophile (3) resulting in a rebridged protein (4). (b) Different bis‐electrophiles have been reported: α,β‐unsaturated‐β’‐monosulfone (3 a), 3,6‐dichloro‐1,2,4,5‐tetrazine (3 b), 3,3‐bis(bromomethyl)oxetane (3 c) to form new disulfide bridges 4 a‐4 c, respectively.
