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Isopeptide bond formation with different pairs of tags: (a) Protein (19, blue) with N‐ and C‐terminal tags containing a lysine (A, orange) and an asparagine or aspartic acid (B, yellow), respectively, is cyclized (20). (b) Table with pairs of tags including the corresponding numbers of involved amino acids. * SpyCatcher and SpyTag have been fused at both N‐ and C‐terminus. # Isopeptide formation requires the presence of SnoopLigase.
