Figure 2.
Hemopoietic and nonhemopoietic cells contribute to IL‐1β production following IAV infection. (a, b) C57BL/6 mice were infected with 105 PFU of HKx31 and CD45+ hemopoietic and CD45– nonhemopoietic cells were isolated from lungs on days 1 and 3 postinfection. Uninfected control mice were included for comparison (day 0). mRNA expression of (a) Nlrp3, Caspase 1, Asc and (b) Il‐1β and Il‐18 relative to Gapdh. Data from two independent experiments were pooled (n = 8 or 9). Individual mice are shown as symbols and bars represent the mean. *P < 0.05, **P < 0.01; one‐way ANOVA. IAV, influenza A virus; IL, interleukin; mRNA, messenger RNA; PFU, plaque‐forming units.