TABLE 3.
PCR primers for identification and characterization of integrons and gene cassettes in V. cholerae O1 from Vietnam
| PCR primer
|
Accession no.b | Primer position | Reference | ||
|---|---|---|---|---|---|
| No. | Namea | Sequence | |||
| 1 | int1 Fa (5′–CS) | GGC ATC CAA GCA GCA AGC | U12338 | 1416→1433 | Collis and Hall (7) |
| 2 | int1 Ba (3′-CS) | AAG CAG ACT TGA CCT GAT | U12338 | 4831→4814 | Hall et al. (13) |
| 3 | qacEΔ1 F | ATC GCA ATA GTT GGC GAA GT | X15370 | 211→230 | Stokes and Hall (40) |
| 4 | sul1 B | GCA AGG CGG AAA CCC GCG CC | X12869 | 1360→1341 | Stokes and Hall (40) |
| 5 | ant(3")-Ia B | ATT GCC CAG TCG GCA GCG | M10241 | 1040→1023 | Hollingshead and Vapnek (17) |
a
F, nucleotide sequence forward 5′→3′; B, nucleotide sequence backward 3′←5′.
b
Accession numbers are from the published sequences in the GenBank database.