FIGURE 8.

The relative content of cyclic nucleotides in the aorta of CSE^−/− mice and WT mice. The cyclic nucleotides levels are detected simultaneously in homogenates of mouse aortas using UPLC‐MS/MS. typical trace analysis of the determination of the cyclic nucleotides in (a) WT and (b) CSE ^−/− mice. The cyclic nucleotide levels in WT are 261.99 ± 9.36 (cAMP), 9.13 ± 0.89 (cGMP), 19.87 ± 6.43 (inosine 3′,5′‐cyclic monophosphate/cIMP) pg/mg tissue. The cyclic nucleotides levels in CSE^−/− mice are 156.38 ± 5.51 (cAMP), 13.31 ± 0.63 (cGMP), 0.18 ± 0.02 (cIMP) pg/mg tissue. (c) the data are expressed as a relative change compared to the levels of cyclic nucleotides detected in WT aorta, taken as 1. Values shown are means ± SEM; n = 5 determinations (three mouse aortas are pulled in each sample). *P < 0.05 significantly different as indicated; one‐way ANOVA with Bonferroni's post hoc test