TABLE 1.

Effect of NaHS on purified soluble guanylyl cyclase (sGC) with or without NO by using either ITP or GTP as substrates

	ITP K m (μM)	ITP V max (μmole min−1 mg−1)
Basal activity	69.3 ± 27.5	0.084 ± 0.009
With 300‐nMNaHS	21.3 ± 8.2*	0.078 ± 0.007
With 300‐μM NaHS	96.9 ± 37.7	0.093 ± 0.013
10‐μM NO	66.4 ± 11.1	2.86 ± 0.12*
With 300‐nMNaHS	62.3 ± 16.5	2.73 ± 0.15
With 300‐μM NaHS	94.2 ± 15.7#	2.66 ± 0.17#

	GTP K m (μM)	GTP V max (μmole min−1 mg−1)
Basal activity	154 ± 13.2	0.099 ± 0.005
With 300‐nMNaHS	299.8 ± 48.9*	0.166 ± 0.020*
With 300‐μM NaHS	149.4 ± 15.1	0.150 ± 0.011*
10‐μM NO	44.4 ± 3.8*	6.39 ± 0.13*
With 300‐nMNaHS	44.0 ± 7.5*	5.69 ± 0.23* , #
With 300‐μM NaHS	87.0 ± 19.2* , #	5.68 ± 0.22* , #

^*Statistically significant vs. basal value.

^# Statistically significant vs. NO‐induced value.