TABLE 1.
Comparison of Rapid NH test reaction results of 65 A. actinomycetemcomitans isolates representing five serotypes with those in the Rapid NH system differential chart for the identification of A. actinomycetemcomitans
| Substrate | Positive identification of A. actinomycetemcomitans according to Rapid NH system differential chart
|
Rapid NH system results for tested A. actinomycetemcomitans isolates (no. [%] of isolates) for:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Reaction | %a | Serotype a (n = 6)b | Serotype b (n = 10) | Serotype c (n = 7) | Serotype d (n = 3) | Serotype e (n = 38) | Nonserotypeable (n = 1) | Total (n = 65) | |
| Proline p-nitroanilide | − | 100 | 1 (17) | 1 (10) | 2 (29) | 0 (0) | 10 (26) | 0 (0) | 14 (22) |
| γ-Glutamyl p-nitroanilide | + | 90 | 5 (83) | 10 (100) | 7 (100) | 3 (100) | 38 (100) | 1 (100) | 64 (98) |
| o-Nitrophenyl-β-d-galactosidase | − | 100 | 6 (100) | 10 (100) | 7 (100) | 3 (100) | 38 (100) | 1 (100) | 65 (100) |
| Glucose | + | 83 | 6 (100) | 10 (100) | 7 (100) | 3 (100) | 38 (100) | 1 (100) | 65 (100) |
| Sucrose | − | 100 | 6 (100) | 10 (100) | 7 (100) | 2 (67) | 21 (55) | 1 (100) | 47 (72) |
| Fatty acid ester | − | 100 | 3 (50) | 2 (20) | 0 (0) | 2 (67) | 16 (42) | 0 (0) | 23 (35) |
| Resazurin | + | 92 | 2 (33) | 4 (40) | 5 (71) | 3 (100) | 13 (34) | 0 (0) | 27 (42) |
| p-Nitrophenyl phosphate | + | 92 | 6 (100) | 0 (0) | 3 (43) | 3 (100) | 35 (92) | 1 (100) | 45 (70) |
| Ornithine | − | 100 | 6 (100) | 10 (100) | 7 (100) | 3 (100) | 38 (100) | 1 (100) | 65 (100) |
| Urea | − | 100 | 6 (100) | 10 (100) | 7 (100) | 3 (100) | 38 (100) | 1 (100) | 65 (100) |
| Nitrate | + | 96 | 5 (83) | 9 (90) | 7 (100) | 3 (100) | 25 (66) | 1 (100) | 50 (77) |
| Tryptophane | − | 100 | 6 (100) | 10 (100) | 7 (100) | 3 (100) | 38 (100) | 1 (100) | 65 (100) |
| No identification | 5 (83) | 10 (100) | 7 (100) | 1 (33) | 31 (82) | 1 (100) | 55 (85) | ||
a
Proportion of strains.
b
Number of isolates tested.