TABLE 3.
Results of API ZYM characterization of clinical A. actinomycetemcomitans isolates representing all serotypes and various Haemophilus speciesa
| Enzyme activity |
Haemophilus spp. (S/W/VW)
|
A. actinomycetemcomitans (S/W/VW)
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H. aphrophilus(n = 11) | H. paraphrophilus(n = 2) | H. influenzae(n = 4) | H. parain-fluenzae(n = 3) | H. haemolyticus(n = 1) | H. parahae-molyticus(n = 1) | H. segnis(n = 1) | Serotype a (n = 6) | Serotype b (n = 10) | Serotype c (n = 7) | Serotype d (n = 2) | Serotype e (n = 11) | Nonsero-typeable (n = 1) | |
| Alkaline phosphatase | 11/0/0 | 2/0/0 | 4/0/0 | 3/0/0 | 1/0/0 | 1/0/0 | 1/0/0 | 6/0/0 | 10/0/0 | 7/0/0 | 2/0/0 | 11/0/0 | 1/0/0 |
| Esterase (C4) | 0/6/5 | 0/2/0 | 0/0/2 | 0/0/3 | 0/0/0 | 0/1/0 | 0/0/1 | 0/6/0 | 0/9/0 | 0/7/0 | 0/2/0 | 0/11/0 | 0/1/0 |
| Esterase lipase (C8) | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/5/1 | 0/7/3 | 0/3/3 | 0/1/1 | 0/7/3 | 0/0/1 |
| Leucine arylamidase | 11/0/0 | 2/0/0 | 4/0/0 | 3/0/0 | 1/0/0 | 1/0/0 | 1/0/0 | 6/0/0 | 10/0/0 | 7/0/0 | 2/0/0 | 11/0/0 | 1/0/0 |
| Valine arylamidase | 1/2/8 | 0/1/1 | 0/4/0 | 0/0/3 | 0/0/0 | 0/0/0 | 0/0/1 | 0/4/2 | 0/3/7 | 0/4/2 | 0/1/1 | 0/9/2 | 0/1/0 |
| Cystine arylamidase | 0/2/6 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/4 | 0/4/3 | 0/1/4 | 0/0/1 | 0/4/1 | 0/0/1 |
| Acid phosphatase | 11/0/0 | 2/0/0 | 4/0/0 | 3/0/0 | 1/0/0 | 1/0/0 | 1/0/0 | 6/0/0 | 10/0/0 | 7/0/0 | 2/0/0 | 11/0/0 | 1/0/0 |
| Naphthol-AS-BI-phosphohydrolase | 3/8/0 | 2/0/0 | 1/3/0 | 3/0/0 | 1/0/0 | 1/0/0 | 0/1/0 | 0/0/6 | 0/2/8 | 0/1/6 | 0/1/1 | 0/6/4 | 0/0/1 |
| β-Galactosidase | 11/0/0 | 2/0/0 | 0/0/0 | 0/2/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 |
| α-Glucosidase | 11/0/0 | 2/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 | 0/0/0 |
a
Sign: S, number of strains with strong reaction (grades 3, 4, and 5 according to color chart: ≥20 nmol); W, number of strains with weak reaction (grades 1 and 2: 5 to <20 nmol); VW, number of strains with very weak reaction (<5 nmols). n, number of isolates tested.